siRNA Delivery via Exosomes

What are Exosomes?

Exosomes are natural carriers for mRNA, lncRNA, miRNA, siRNA, protein, DNA, and peptides for intercellular communication. Exosomes are an ideal vehicle for intracellular targeted therapeutics delivery, especially for siRNA (Alvarez-Erviti et al., 2011). The endosomal membrane origination property allows exosomes to escape from endosome trappings and release its cargo into cytosol for its biological function (Zheng et al., 2019). To enhance its specific cancer cell targeting property, we utilize an arrow shaped RNA nanoparticle to display a ligand onto the surface of the exosomes. Decorating these exosomes with RNA nanoparticles that harbor aptamers or chemical ligands against different cancer cell surface markers enhance its biodistribution to the cancer cells after systemic administration (Pi et al., 2018).

In vivo properties of siRNA loaded exosomes can be finely tuned through decorating the exosomes’ surface of RNA Nanoparticles conjugated with different kinds of ligands. An example of this would be displaying the EGFR aptamer to enhance its biodistribution to EGFR positive breast cancer cells, or PSMA aptamer for prostate cancer cells, or folate for folate receptor overexpressing cancer cell, etc.

ExonanoRNA provides the following tested products cataloged below for your use.

1. siRNA to be loaded into exosomes
2. ligand to display on the exosomes

For customized projects, please contact us and provide us with the following information and we will provide you with the right solution and products for your research. Contact us at [email protected]

Survivin siRNA Delivery via Exosomes

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Product Name	Size	Catalog No.
Breast Cancer Targeting Survivin siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting breast cancer cells	1 nmol	EXO-siSUR-1
	50 nmol	EXO-siSUR-2
Prostate Cancer Targeting Survivin siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells	1 nmol	EXO-siSUR-3
	50 nmol	EXO-siSUR-4
Gastric Cancer Targeting Survivin siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells	1 nmol	EXO-siSUR-5
	50 nmol	EXO-siSUR-6
Liver Cancer Targeting Survivin siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Liver cancer cells	1 nmol	EXO-siSUR-7
	50 nmol	EXO-siSUR-8

Breast Cancer Targeting Survivin siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting breast cancer cells

Size	Catalog No.
1 nmol	EXO-siSUR-1
50 nmol	EXO-siSUR-2

Prostate Cancer Targeting Survivin siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells

Size	Catalog No.
1 nmol	EXO-siSUR-3
50 nmol	EXO-siSUR-4

Gastric Cancer Targeting Survivin siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells

Size	Catalog No.
1 nmol	EXO-siSUR-5
50 nmol	EXO-siSUR-6

Liver Cancer Targeting Survivin siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Liver cancer cells

Size	Catalog No.
1 nmol	EXO-siSUR-7
50 nmol	EXO-siSUR-8

Luciferase siRNA Delivery via Exosomes

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Product Name	Size	Catalog No.
Breast Cancer Targeting Luciferase siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting breast cancer cells	1 nmol	EXO-siLUC-1
	50 nmol	EXO-siLUC-2
Prostate Cancer Targeting Luciferase siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells	1 nmol	EXO-siLUC-3
	50 nmol	EXO-siLUC-4
Gastric Cancer Targeting Luciferase siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells	1 nmol	EXO-siLUC-5
	50 nmol	EXO-siLUC-6
Liver Cancer Targeting Luciferase siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Liver cancer cells	1 nmol	EXO-siLUC-7
	50 nmol	EXO-siLUC-8

Breast Cancer Targeting Luciferase siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting breast cancer cells

Size	Catalog No.
1 nmol	EXO-siLUC-1
50 nmol	EXO-siLUC-2

Prostate Cancer Targeting Luciferase siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells

Size	Catalog No.
1 nmol	EXO-siLUC-3
50 nmol	EXO-siLUC-4

Gastric Cancer Targeting Luciferase siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells

Size	Catalog No.
1 nmol	EXO-siLUC-5
50 nmol	EXO-siLUC-6

Liver Cancer Targeting Luciferase siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with firefly luciferase siRNA and decorated with RNA nanoparticles targeting Liver cancer cells

Size	Catalog No.
1 nmol	EXO-siLUC-7
50 nmol	EXO-siLUC-8

Custom siRNA Delivery via Exosomes

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Product Name	Size	Catalog No.
Breast Cancer Targeting Custom siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with custom siRNA and decorated with RNA nanoparticles targeting breast cancer cells	1 nmol	EXO-sixxx-1
	50 nmol	EXO-sixxx-2
Prostate Cancer Targeting Custom siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with custom siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells	1 nmol	EXO-sixxx-3
	50 nmol	EXO-sixxx-4
Gastric Cancer Targeting Custom siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with custom siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells	1 nmol	EXO-sixxx-5
	50 nmol	EXO-sixxx-6
Liver Cancer Targeting Custom siRNA Delivery via Exosomes HEK293T cells derived exosomes loaded with custom siRNA and decorated with RNA nanoparticles targeting Liver cancer cells	1 nmol	EXO-sixxx-7
	50 nmol	EXO-sixxx-8

Breast Cancer Targeting Custom siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting breast cancer cells

Size	Catalog No.
1 nmol	EXO-sixxx-1
50 nmol	EXO-sixxx-2

Prostate Cancer Targeting Custom siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Prostate cancer cells

Size	Catalog No.
1 nmol	EXO-sixxx-3
50 nmol	EXO-sixxx-4

Gastric Cancer Targeting Custom siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Gastric cancer cells

Size	Catalog No.
1 nmol	EXO-sixxx-5
50 nmol	EXO-sixxx-6

Liver Cancer Targeting Custom siRNA Delivery via Exosomes

HEK293T cells derived exosomes loaded with human survivin siRNA and decorated with RNA nanoparticles targeting Liver cancer cells

Size	Catalog No.
1 nmol	EXO-sixxx-7
50 nmol	EXO-sixxx-8

References:

Alvarez-Erviti, L., Seow, Y., Yin, H., Betts, C., Lakhal, S., and Wood, M.J. (2011). Delivery of siRNA to the mouse brain by systemic injection of targeted exosomes. Nat Biotechnol 29, 341-345.

Pi, F., Binzel, D.W., Lee, T.J., Li, Z., Sun, M., Rychahou, P., Li, H., Haque, F., Wang, S., Croce, C.M., et al. (2018). Nanoparticle orientation to control RNA loading and ligand display on extracellular vesicles for cancer regression. Nature nanotechnology 13, 82-89.

Zheng, Z., Li, Z., Xu, C., Guo, B., and Guo, P. (2019). Folate-displaying exosome mediated cytosolic delivery of siRNA avoiding endosome trapping. J Control Release 311-312, 43-49.