Exojuice
Exosome Purification & Concentration System
Details:
Exojuice - Exosome Purification & Concentration System is a scientific research reagent designed to improve the purity of exosomes by ultracentrifugation. The reagent can be used as a cushion to purify exosomes from cell culture supernatants or other media, and for virus biomotor purification. It is safe, sterile, isotonic, and easy to remove. Exojuice is easy to use, easy to operate, and reduces the cumbersome process of collecting exosomes after over-distribution.
Advantages of Exojuice
The use of Exojuice makes it easier to purify exosomes compared to the traditional methods listed above. Comparatively, Exojuice: 1. Can obtain a high purity of intact exosomes by ultracentrifugation 2. Is non-toxic and safe, and it can be removed by simply dialysis 3. Has a low osmotic pressure to maintain the structure of the exosomes 4. Has a low molar concentration, molecular weight, and viscositySpecifications:
Catalog Number: EXO-008
Use: Purification of Exosomes
Storage: 4 ℃ for 1 year
| Catalog No. | No. of Applications | Price USD ($) | Buy |
| EXO-008-01 | 20 | 214 | Buy Now |
| EXO-008-02 | 60 | 428 | |
| EXO-008-03 | 200 | 714 |
Exojuice Workflow:
The diagram below shows a typical workflow of Exosome isolation using Exojuice™ from cell culture medium.
Instructions for Use:
1. Harvest cell culture medium and use centrifuge at 12,000 g for 30 min to recover the supernatant for the next step. This step is to remove cell debris from the cell culture medium.
2. Put the supernatant from step 1 in an ultracentrifuge tube and add 200 µL of Exojuice reagent to the bottom of the centrifuge tube. After using the centrifuge at 100,000 g for 70 min, collect the bottom 500 µL of liquid. When using the SW28 rotor for this step, about 230 mL of the culture supernatant will be concentrated to 3 mL in one run.
3. Mix 3 ml of crudely extracted exosomes to 2.5 mL of sterile PBS (pH 7.2, filtered through a 0.22 μm filter), then add it to a centrifuge tube. Then add 500 µL of Exojuice to the bottom of this tube. After using the centrifuge at 100,000 g for another 70 min, take out the centrifuge tube and slowly draw 300 µL of liquid (1) from the bottom, discard it, and collect 200 µL of liquid (2) for a higher purity exosomes.
4. Dialysis can be performed to remove Exojuice reagent from the purified exosomes with dialysis membrane with 1KD cutoff. Add 200 µL of exosome extract to the dialysis bag against 100 mL of 1 x PBS buffer for 2 hr. The liquid in the bag to collect is purified exosomes (this step can be operated according to the purpose of the experiment). Exosomes can be obtained for subsequent proteome, transcriptome, metabolome, sequencing and other analyses.
Comparison of Exojuice™ to Optiprep™ as a cushion for exosome isolation from cell culture supernatant
A.) Better Migration Pattern in Ultracentrifugation:
Optiprep™ purified exosomes travel to the interface of the cushion while Exojuice™ purified exosomes form a tight band inside the Exojuice™ layer.
B.) Higher surface marker concentration in Western Blot:
Exosomes purified with Exojuice™ show a higher concentration of protein markers in western blot compared to Optiprep™ purified samples
C.) Narrow size distribution and a high degree of intact exosomes in Atomic Force Microscopy images show that Exojuice™ purification retains the native shape of exosomes better than Optiprep™
