ChemRNA
ChemRNA - RNA Transcription Kit
Details:
ChemRNA – This transcription kit contains a recombinant mutant form of T7 polymerase, which can incorporate modified or normal ribonucleoside triphosphates (NTPs) to RNA along a DNA template with a T7 promoter. The transcribed modified RNA products are resistant to RNase A degradation. This kit is suitable for RNA aptamer synthesis and produces RNA for RNAi, gRNA, or antisense RNA experiments.
The ChemRNA Transcription Kit is ready to use.
Specifications:
Contents: ChemRNA T7 polymerase, 10x reaction buffer, ATP, GTP, 2’F-CTP, 2’F-UTP, 0.1M DTT, and DEPC treated water.
Use: High Yield in vitro Transcription, RNase-Free
Storage: -20 ℃ for 1 year
| Catalog No. | Kit Size | Price USD ($) | Buy |
| MT7-10 | 10 rxns | 500 | Buy Now |
| MT7-30 | 30 rxns | 1,286 |
Current difficulties encountered in RNA research
The RNA transcripts from standard NTPs can be easily degraded by the RNase in the experimental environment; however, ChemRNA Transcription Kit can avoid that.
1. The RNA research requires the target RNA can be easily accessed. Long-length RNA oligonucleotide (nt > 150) mostly cannot be synthesized via the chemical approach due to the technical difficulties and high cost. ChemRNA Transcription Kit can solve the problem since we tested the kit could transcribe RNA transcripts up to 1400 nt.
2. Standard RNA transcripts that are catalyzed from a traditional T7 RNA polymerase can be easily degraded by the RNase. Our kit can incorporate modified NTPs to RNA, thus the RNA transcripts are highly stable and resistant to RNase degradation.
Advantages of ChemRNA transcription kit
Compared to the same type of products on the market, the ChemRNA Transcription Kit has:
1. The core - a high-activity mutant T7 RNA Polymerase that produces high RNA yields.
2. The stable RNA transcripts that are resistant to RNase degradation.
3. The long-length RNA transcripts (up to 1400 nt as we tested).
Instructions for Use:
Take out all the reagents from the freezer, thaw on ice, and mix the reaction components at room temperature in the following:
| DNA template | >1.2 µg |
| ATP (50 mM) | 2.5 µl |
| GTP (50 mM) | 2.5 µl |
| 2′-F-CTP (50 mM) | 2.5 µl |
| 2′-F-UTP (50 mM) | 2.5 µl |
| 10×Reaction Buffer | 2.5 µl |
| DTT (100 mM) | 2.5 µl |
| Nuclease-Free Water, Sterile | x |
| Mutant T7 RNA Polymerase | 2.5 µl |
| Total | 25 µl |
The whole reaction system can be expanded if necessary. Incubate the reaction at 37 °C overnight. After the reaction is complete, add 1 μL of RNase-Free DNase I (1 mg/ml) to each 25 μL reaction. Incubate at 37 °C for 30 minutes to stop the reaction, if there’s any DNA template residue, add more DNase I. The RNA transcripts are resistant to DNase I and RNase A digestion. The targeted RNA product can be further purified by gel purification if needed.
Compared with commercial L kit on the market
ChemRNA Transcription kit produces higher RNA transcripts products under the same experimental conditions: **
* The synthesis was tested using a DNA template (> 3 kbp) with a T7 promoter and modified NTPs following our standard protocol.
** The comparative experiment was done using a 58 bp DNA template with a T7 promoter and modified NTPs under the exact same conditions. Equal amounts of RNA transcript products were loaded to gel for analysis.
