Rna and Dna Oligos

ExonanoRNA is committed to supplying high-quality oligonucleotides for use in research, therapeutic, and diagnostic applications at competitive prices. We specialize in the production of oligonucleotides with challenging modifications and offer quantities ranging from .2umol to 1mmol. Our oligonucleotides can be purified as requested, whether that be standard desalting, anion-exchange HPLC, ion-paired reversed phase HPLC, or PAGE purification. All oligonucleotides are deprotected and shipped ready to use. Most orders can be shipped within weeks of order placement.

Recent publication from our customer: Mono- and bilayer smectic liquid crystal ordering in dense solution of “gapped” DNA duplexes, PNAS, Mar 23, 2021 118(12) e201996118.  [LINK]

How to Order

1. Download our Oligo Ordering Form Excel File and fill it out.

2. Email it back to us at support@exonanorna.com.

You can also directly email us with any other questions you may have.

Pricing Chart

(Prices Listed in USD)

Custom Oligonucleotides.3 µmol1 µmol3 µmol10 µmol
DNA (per base)1.5024.9012.60
RNA (per base)8.501126.7069.30
2’O-Methyl RNA (per base)8.501126.7069.30
2’F RNA (per base)11143488.20
BNA (per base)3645109.40283.50
Setup fee (per ONT)75757575
HPLC purification100150200300
PAGE purification125175250600

Customization Details

Custom RNA – RNA

ExonanoRNA is specially equipped to provide customized targeting aptamers, RNA therapeutics (such as siRNA, miRNA, anti-miRNA), oligonucleotide probes, and custom oligos for other applications. We also offer (a) 2’F, BNA, or 2’O-methyl modifications enhanced nuclease stability (b) highly sensitive fluorophores to either end of your oligonucleotide for use as chemical probes. Need to test the effect of a small molecule? (c) amino modifiers to assist your downstream bioconjugation reaction to help test the effect of a small molecule. These are just some of the modifications we can introduce for your desired application.

2’F RNA Hybrids – Modified Oligos

2’ Fluorine modified oligonucleotides have unique properties rendering them useful for a variety of applications. They show greater nuclease resistance, increased pairing efficacy in vivo and in vitro, and lower immunostimulatory behavior compared to unmodified siRNA. (Pallan, 2011) By using 2’F modified ribo phosphoramidites during synthesis, modified oligonucleotides can be produced without a change in sequence.

Bridged Nucleic Acids – Modified Oligos

Bridged Nucleic acids (BNA’s) contain five, six, or seven-membered bridge structures synthetically incorporated between the 2’ and 4’ position of an oligonucleotide monomer. The ribose residue exists in an inflexible locked N-type conformation that promotes DNA or RNA hybridization. These oligonucleotides show increased melting temperatures, enhanced nuclease resistance, and increased binding capacities for antisense applications. They can be incorporated into ribonucleotides, deoxyribonucleotides, and other oligomer analogs at any desired position.

2’O-Methyl Oligos – Modified Oligos

2’ O-methyl modifications can be found in nature, mostly within essential areas of ribosomal RNA and small nuclear RNA. This modification has also found various uses when incorporated into synthetic oligonucleotides. Like many other developed modifications, they can increase nuclease resistance, enhance binding affinity, and reduce immune stimulation. The enhanced Tm upon RNA binding allows for efficient binding to duplex RNAs. By using 2’-O methyl ribophosphoramidites, these modifications may be incorporated at any position.

Hybrid Oligos – Modified Oligos

Oligonucleotides can be produced with a combination of RNA, DNA, and modified bases (2’-F, 2’-O-Methyl, BNA) to serve your research needs.

Amine Modifications

A primary amino group can be incorporated into the 3’, 5’, or both ends of an oligonucleotide during synthesis. Amine functionalization promotes flexible bioconjugation chemistry with a diverse range of molecules.

Click chemistry modifications

Click chemistry is a highly efficient coupling technique that utilizes the specific reactivity of alkynes with azides in the presence of a copper (I) catalyst. Due to its selectivity, biocompatibility, stereospecificity, and coupling efficiency, it has found increasing uses in nucleic acid research. (Brechbiel 2009) Our oligonucleotides can be functionalized with azides or alkynes through a two-step process consisting of synthesis with an amino modifier at either the 3’ or 5’ end, and then NHS coupling with either NHS-alkyne or NHS-azide. The final product is a highly pure oligonucleotide ready for use in your click conjugation.

Fluorophore modifications

Fluorescent dyes can be incorporated into oligonucleotides either during synthesis or post-synthetically using amine modifications, depending on their stability to synthesis and deprotection conditions. These molecules have been used as probes in applications such as STR analysis, TaqMan assays, fluorescence In-situ hybridization (FISH), and biodistribution studies. We offer a variety of different fluorophores, while the most commonly used are IF647 and Cy5.

Other terminal modifications

A multitude of modifications can be incorporated into the 3’ or 5’ end of custom oligonucleotide sequences to be used as targeting agents, chemical probes, or reactive linkers for bioconjugations. Listed below are some of the modifications we specialize in at ExonanoRNA, while many others are available upon request.

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Specifications

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